Regulatory

Part:BBa_K339006:Design

Designed by: Christopher Tang   Group: iGEM10_Calgary   (2010-10-25)

CpxP Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cpxP promoter was extracted from the E. coli genome through PCR using primers designed by literature (Shimohata et al. 2002). The XbaI restriction site was added in front of the gene and the full Biobrick suffix (SpeI, NotI, and PstI cut sites) were added to the end.

Source

The primer sequences used to isolate cpxP from the E. coli genome were 5′-CCGGAATTCTGTTTAAATACCTCCGAGGC (Forward) and 5′-CGCGGATCCGCTCCCAAAATCTTTCTGTCG (Reverse). (Shimohata et al. 2002)

References

Shimohata, N., Chiba, S., Saikawa, N., Ito, K., and Akiyama, A., 2002., The Cpx stress response system of Escherichia coli senses plasma membrane proteins and controls HtpX, a membrane protease with a cytosolic active site. Genes to cells 7(7):653-662